Composition For Enhancing Protein Strength

ABSTRACT

A composition for enhancing protein strength according to the present invention contains an aminosilane compound capable of covalent binding with a protein of hair, scalp, skin, nails, leather, or textile, so that the protein and the amino silane compound form a covalent bond, and thus the composition can improve the protein strength enhancement effect and maximize the semi-permanent protein strength enhancement effect.

CROSS-REFERENCE TO RELATED APPLICATIONS

This application is a national phase entry under 35 U.S.C. § 371 ofInternational Application No. PCT/KR2017/014555, filed Dec. 12, 2017,which claims priority to Korean Patent Application No. 10-2017-0004038,filed Jan. 11, 2017 and Korean Patent Application No. 10-2017-0161920,filed Nov. 29, 2017.

TECHNICAL FIELD

The present invention relates to a composition for enhancing proteinstrength of hair, skin, finger/toe nails, leather or fiber.

BACKGROUND ART

The skin is tissue covering the surface of a body, consisting of theepidermis, the dermis and a subcutaneous fat layer, and skin appendagesinclude a sweat gland, a sebaceous gland, hair, and hand/toe nails. Theepidermis constituting the outermost part of the skin is derived fromthe ectoderm, and largely consists of an inner part formed of livingcells called the malpighian layer, and an outer part, the stratumcorneum, formed of anucleate, flat, dry and dead cells. The thicknessesof the skin are about 0.03 mm at a face part, about 0.16 to 0.8 mm atthe palms and soles of feet, and about 0.1 mm at an eyelid, which is thethinnest part. The epidermis is generally the stratified squamouscornified epithelium, mainly consisting of keratinocytes, and alsoincluding melanocytes, Langerhans cells and Merkel cells.

The keratinocyte is the main component of the epidermis, which isdivided into four layers such as the cornified layer (the outermostlayer), the granular layer, the spinous layer and the basal layer, andthere is a translucent layer between the cornified layer and thegranular layer at the soles of palms and feet.

Millions of new cells are formed every day in the epidermis, andcontinue to be shed from the outermost part of the epidermis withoutstaying in one place. Here, these cells gradually turn into rigidkeratin when being shed from the cells. The cells of the epidermis arefirst made in the basal layer, and their shapes and functions are alsochanged while gradually rising toward the surface.

The epidermis is gradually transformed into the basal layer, the spinouslayer, the granular layer and the cornified layer as keratinized, andall of these cells are cells made during keratogenesis, and thus calledkeratinocytes.

A differentiation process in the keratinocytes is performed through foursteps: 1) division of basal cells, 2) synthesis and alignment in spinouscells, 3) self-degradation in granular cells, and 4) reconstruction incorneocytes, and in the final step of differentiation, the cornifiedlayer is formed. This process is called keratinization.

The life span of keratinocytes is about 28 days, and although slightlyvarying from site to site, millions of keratinocytes are detached everyday, and millions of new cells are generated from the underlying layer.On the skin surface of the human body, aging keratinocytes arecontinuously being shed, and in aged skin, it takes more time for thedetachment of the cornified layer, resulting in thickening of thecornified layer. Therefore, the functional degradation of keratinocytesleads to generation of more dead cells, and causes fine lines and skinroughness.

The cornified layer is a part forming keratin, which is the outermost,rigid, dry and thin layer, and consisting of 20 to 25 layers. Thecornified layer is the name given due to the fact that a proteinconsists of keratin made by changing a protein to be rigid. As it isclose to the skin surface, the cornified layer has a flat and longshape. The main components of the cornified layer include the keratinprotein (58%), a lipid (11%), a natural moisturizing factor (NMF, 38%).The thickness of the cornified layer varies depending on a body part,and the palms or soles have very thick cornified layers and thus theycan withstand physical impact, friction or trauma which is received indaily life. Although the cornified layer consists of anucleate, deadcells and keratin protein, it is most important in terms of skinprotection. The cornified layer plays an important role in preventingexternal bacteria or foreign toxic substances from entering the body,and dehydration of the body. The main component of the cornified layeris the keratin protein (58%), consisting of amino acids such as glycine,alanine, valine, leucine, isoleucine, threonine, serine, cysteine,cystine, methionine, aspartic acid, asparagine, glutamic acid,diiodotyrosine, lysine, arginine, histidine, phenylalanine, tyrosine,tryptophan, proline and oxyproline, and particularly, the keratinconstituted in the cornified layer includes about 6.4 to 8.1% ofaspartic acid, about 9.1 to 15.4% of glutamic acid, and about 3.1 to6.9% of lysine.

Generally, hair is a keratinized structure formed by embryologicaldepression of the epithelium, and corresponds to one of the skinappendages including a sweat gland and a sebaceous gland. Hair refers tothe coat covering the outside of the human body, including the hair onthe head, soft hair, mustaches or beards, the hair under the arms, pubichair, etc., and in some cases, only refers to hair on the scalp.

Hair consists of the keratin protein which is the same as the maincomponent of the cornified layer, and keratin is largely classified intotwo according to origin. The keratin constituting the cornified layer iscalled soft keratin, and keratin constituting hair and finger nails iscalled hard keratin. The keratin is divided due to the difference incontent of cysteine, which is one of the amino acids, and the hardkeratin has a high cysteine content, and the soft keratin has a lowcysteine content. Therefore, the hard keratin is highly resistant to anexternal stimulus or the penetration of a chemical substance.

A hair fiber has a thickness of about 50 to 100 μm, consists of acuticle and a cortex, and sometimes, further includes a medulla, presentin the middle of the hair fiber. All of these cells are dead cells, andmost of them are filled with the keratin protein.

Human hair consists of about 65 to 95% of the keratin protein, andincludes water and lipids (binding and unbinding types), a pigment, andtrace elements. The protein is formed of mixtures of about 20 to 50amino acids in a long chain, and each chain is wound in a helical orcoiled form.

Among the numerous amino acid compositions constituting human hair,cystine is one of the most important amino acids. Cystine is composed oftwo cysteines (thiols), which are present in different amino acidchains, respectively, and has a disulfide bond known as a very strongbond forming a bond between two sulfur atoms in close proximity to eachother. In addition to the disulfide bond, hair includes very abundantpeptide bonds. In addition, due to the presence of many CO—and NH—groups, there is hydrogen bonding between neighboring chain moleculargroups. However, due to a high cystine content, which is the very uniquepart of the cell structure of human hair, the physical characteristicsof hair are exhibited. A pigment, which is a part related to a haircolor, is melanin, and is mainly present in granule form at the cortexof human hair.

Most of the hair consists of a protein, and the other components includethe melanin pigment, lipids, trace elements, and water. The protein thatconstitutes most of the amino acid composition of hair is the keratinprotein abundantly containing cysteine. Hair keratin consists of about18 types of amino acids, and compared to the human epithelium, it has alarge cysteine content. In addition, a ratio of basic amino acids, forexample, histidine, lysine and arginine is 1:3:10, which is the uniqueratio of hair keratin. Skin collagen has high glycine, proline andalanine contents without cysteine. The melanin pigment determining haircolor is contained in the hair at about 3% or less.

In addition to the protein and the melanin pigment, it has been knownthat the hair contains minerals such as copper, zinc, iron, calcium andmagnesium, and trace elements, and other than these elements, alsocontains inorganic components such as phosphorus, silicon, etc.

In addition to the peptide bonds between amino acids in the protein,bonds present in the hair include intermolecular forces or bindingstrength between molecules of each protein, which maintain themorphology and shape of hair. Types of bonds largely includecrosslinking bonds such as a cystine bond and a peptide bond, andnon-cros slinking bonds such as an ionic bond, a hydrogen bond and ahydrophobic interaction. Among these, the cystine bond (disulfide bond;—CH₂—S—S—CH₂—), uniquely formed in a sulfur (S)-containing protein, is aside chain bond not shown in other fibers and a bond characteristicallyshown in keratin. The cystine bond imparts strong physiochemicalcharacteristics to the keratin.

The keratin protein (58%), which is the main component of hair, consistsof amino acids such as glycine, alanine, valine, leucine, isoleucine,threonine, serine, cysteine, cystine, methionine, aspartic acid,asparagine, glutamic acid, diiodotyrosine, lysine, arginine, histidine,phenylalanine, tyrosine, tryptophan, proline and oxyproline, andparticularly, the keratin constituting the hair includes about 3.9 to7.7% of aspartic acid, about 13.6 to 14.2% of glutamic acid, and about1.9 to 3.1% of lysine.

Generally, fibers may be largely divided into natural fibers andartificial fibers, and among the natural fibers, a fiber obtained froman animal is referred to as an animal fiber, whose chemical component isa protein, and thus is also called a protein-based fiber. Theprotein-based fibers include a fiber obtained from the coat of an animaland a fiber obtained from cocoons, and the fibers obtained from theanimal coat may be divided into wool obtained from the coat of sheep anda hair fiber obtained from goats, camels, rabbits, horses, cows, orother animals

Like the hair, these protein-based fibers consist of amino acids such asglycine, alanine, valine, leucin, isoleucine, threonine, serine,cysteine, cystine, methionine, aspartic acid, asparagine, glutamic acid,diiodotyrosine, lysine, arginine, histidine, phenylalanine, tyrosine,tryptophan, proline and oxyproline, and the most generally and widelyused wool among the protein-based fibers is known to have an amino acidcomposition similar to the hair.

These protein-based fibers, hair, skin, finger/toe nails and leather aretypes of very rigid proteins under mild conditions such as water or aneutral solvent, but may be easily damaged by physical/chemicaltreatment and environmental stress such as combing, heat of a dryer,dyeing or a permanent, which commonly occurs in common life, physicalfriction in daily life, washing, sunlight, heat of ironing, adsorptionof a pollutant, exposure to ultraviolet rays, exposure to high and lowtemperatures, seawater, or chlorine of a pool. Accordingly, the damagedhair, skin, finger/toe nails, fibers or leather have pores due to therelease of proteins inside and outside and thus becomes rough, therebyhaving problems of decreased softness and elasticity, difficult handlingdue to increased friction, lower glossiness and a turbid color.

Therefore, to improve the surface characteristics of a substrate such ashair, skin, fibers or leather, and thus maintain beautiful and heathyappearance, a treatment agent is used. Particularly, glossiness,softness and smoothness are important and representative surfacecharacteristics to be improved.

Examples of the treatment agents for imparting strength improvementinclude hair cosmetic agents, such as a shampoo, a rinse, a treatment, awax, a spray, a mousse, a hair lotion, a hair cream, a pack, a mask, asheet, etc., cosmetic agents for skin, such as a skin, a lotion, anessence, a serum, a cream, a gel, a foundation, a powder, a makeup base,point makeup, a mask, a patch, etc., fabric care agents such as a fabricsoftener, a fabric coloring agent, a detergent, a treatment, a pre/aftertreatment agent, a laundry aid, a spot stain remover, a spray, etc. Thetreatment agents also include finger/toe nail treatment agents such as apolisher, a nutrient, an enhancer, etc., and a leather treatment agentsuch as a cream, a lotion, an essence, a serum, a gel, a wax, a spray, acleanser, a cleaner, a spot stain remover, a salve-type agent, apolisher, a strip, a sheet, etc.

In addition, generally, the “cosmetic” is used to clean and make thebody look more beautiful, enhance attractiveness, change an appearance,or healthily maintain skin or hair by applying, spraying, or othersimilar methods onto the body, and defined as being slightly effectiveon the human body. Such cosmetics have various functions for keepingvarious body parts, such as the skin, hair, and finger/toe nails,healthy and beautiful, and among these functions, strength improvementmay be one of the main functions of the cosmetics.

General raw materials constituting the cosmetic for improving strengthinclude oil raw materials such as oil, wax, hydrocarbons, higher fattyacids, higher alcohols, ester oil, silicone oil, etc., anionic,cationic, amphoteric or non-ionic surfactants, high-molecular compoundssuch as moisturizing agents, thickening agents and coating agents, UVabsorption/blocking agents, antioxidants, sequestering agents, colorantsincluding dyes and pigments, fragrances, and preservatives.

Other than these materials, as components for exhibiting specialperformance, oil and natural or synthetic fatty acids, fatty alcohols,alcohols, alkylglycerylethers, esters, hydrocarbons, silicones, fluorinecompounds, polyhydric alcohols, saccharides, natural or syntheticpolymers, wax, vitamins, hormones, amino acids, peptides, proteins,animal/plant extracts, mineral extracts, and derivatives thereof may beincluded.

However, most of the components for improving strength included in thiscomposition impart their effect through simple adsorption, rather thanstrong covalent bonding. Due to the various external environments thatare experienced in daily lives after the treatment of the composition,these components are continuously released, and strength is graduallylowered, and during a cleansing process, such a phenomenon is furtheraccelerated.

For example, in Korean Unexamined Patent Application Publication No.2014-0096053, a method for glossy makeup through coating with a keratinsubstance (particularly, for nails) including an alkoxy silane and watercontained at a predetermined molar ratio obtained using a specificequation, and a related kit composition are disclosed. However, due tothe limitation in that only one part of the silane molecule is bound tothe protein surface through dehydration, the composition has a coatingability, but is not effective in strength improvement which can beobtained by binding between the silane molecule and the protein at bothends.

DISCLOSURE Technical Problem

The present invention is directed to providing a composition forenhancing protein strength, which is effective in semi-permanentimprovement in the strength of hair, scalp, skin, finger/toe nails,leather or fiber.

The present invention is also directed to providing a care productincluding the composition for enhancing protein strength.

Technical Solution

To solve the problems of the present invention, the present inventionprovides a composition for enhancing protein strength, which includes anaminosilane compound and a reaction mediator.

In addition, the present invention provides a care product including thecomposition.

Advantageous Effects

A composition for enhancing protein strength according to the presentinvention contains an aminosilane compound which enables covalentbonding with a protein of hair, scalp, skin, finger/toe nails, leatheror fiber, thereby forming a covalent bond between the protein and anaminosilane compound, and thus a protein strength enhancing effect maybe improved, and a semi-permanent protein strength enhancing effect canbe maximized

MODES OF THE INVENTION

The present invention relates to a composition for enhancing proteinstrength, which includes an aminosilane compound and a reactionmediator.

The “composition for enhancing protein strength” used herein refers to acomposition acting to semi-permanently link proteins through covalentbonding between an aminosilane compound and carboxyl groups and aminegroups, which are present in large amounts as a constituent component ofa hair, scalp, finger/toe nail, skin or fiber protein, to enhanceprotein strength. In addition, the composition for enhancing proteinstrength refers to a composition which makes a protein appear thicker,and has side effects such as increasing elasticity and thickness.

The “composition for enhancing protein strength” may include acomposition for enhancing the strength of a hair protein, a compositionfor enhancing the strength of a scalp protein, a composition forenhancing the strength of a skin protein, a composition for enhancingthe strength of a finger/toe nail protein, a composition for enhancingthe strength of a leather protein and/or a composition for enhancing thestrength of a fiber protein.

The composition for enhancing protein strength according to the presentinvention may allow a reaction between a substrate consisting of aprotein and an aminosilane compound using a reaction mediator to form acovalent bond between a carboxyl group of the protein substrate and anamine group of the aminosilane compound, between an amine group of theprotein substrate and a carboxyl group of the aminosilane compoundand/or between the amine group of the protein substrate and an aminegroup of the aminosilane compound, and thus a protein strengthenhancement effect may be semi-permanently maximized

The “substrate consisting of a protein” includes, for example, hair,scalp, skin, finger/toe nails, leather and fiber, but the presentinvention is not limited thereto.

The “aminosilane compound” used herein includes mono-, di- andtri-alkoxyaminosilane compounds represented by the following Formulas 1to 3, which have at least one or more amine groups in the same moleculeas well as forming one to three hydroxyl groups directly binding tosilicon (Si) by hydrolysis with water.

In Formulas 1 to 3, R₁ is each independently hydrogen; or a linear,branched or cyclic hydrocarbon having 1 to 500 carbon atoms or a benzenering hydrocarbon, which includes one or more double bonds in a part ofthe molecule, or is substituted with one or more atoms selected from thegroup consisting of O, N, S, P and Si or substituted in an anionic,cationic or amphoteric form, or includes a structure to which a metalion is bonded in a salt form; and R₂ is each independently a linear,branched or cyclic hydrocarbon having 1 to 500 carbon atoms or a benzenering hydrocarbon, which includes one or more double bonds in a part ofthe molecule, or is substituted with one or more atoms selected from thegroup consisting of O, N, S, P and Si or substituted in an anionic,cationic or amphoteric form, or includes a structure to which a metalion is bonded in a salt form and in which at least one primary orsecondary amine is included at an end of the molecular structure.

The aminosilane compound may be one or more selected from the groupconsisting of 3-aminopropyltriethoxysilane,bis[(3-triethoxysilyl)propyl]amine, 3-aminopropyltrimethoxysilane,4-aminobutyltriethoxysilane, bis[(3-trimethoxysilyl)propyl]amine,3-aminopropylmethyldiethoxysilane, 3-aminopropyldimethylethoxysilane,3-aminopropylmethyldimethoxysilane,aminoethylaminopropyltrimethoxysilane,aminoethylaminopropyltriethoxysilane,aminoethylaminopropylmethyldimethoxysilane,diethylenetriaminopropylmethyldimethoxysilane,piperazinylpropylmethyldimethoxysilane,(n-phenylamino)methyltrimethoxysilane,(n-phenylamino)methyltriethoxysilane,3-(n-phenylamino)propyltrimethoxysilane,n-(n-butyl)-3-aminopropyltrimethoxysilane, 4-aminobutyltriethoxysilane,m-aminophenyltrimethoxysilane, p-aminophenyltrimethoxysilane,aminophenyltrimethoxysilane, m-aminophenyltriethoxysilane,p-aminophenyltriethoxysilane, aminophenyltriethoxysilane,3-aminopropyltris(methoxyethoxy-ethoxy)silane,11-aminoundecyltriethoxysilane,3-(m-aminophenoxy(propyltrimethoxy-silane), aminopropylsilanetriol,3-aminopropylmethyldiethoxysilane,3-aminopropyldiisopropylethoxy-silane,3-aminopropyldimethylethoxysilane,n-(2-aminoethyl)-3-aminopropyltri-methoxysilane,n-(2-aminoethyl)-3-aminopropyltri-ethoxysilane,n-(6-aminohexyl)aminomethyl-triethoxysilane,n-(6-aminohexyl)aminopropyl-trimethoxysilane,n-(2-aminoethyl)-11-aminoundecyl-trimethoxysilane,(aminoethylaminomethyl)phenethyl-trimethoxysilane,n-3-[(amino(polypropylenoxy)]amino-propyltrimethoxysilane,n-(2-aminoethyl)-3-aminopropyl-silanetriol,n-(2-aminoethyl)-3-aminopropylmethyl-dimethoxysilane,n-(2-aminoethyl)-3-aminoisobutyl-methyldimethoxysilane,(aminoethylamino)-3-isobutyldi-methylmethoxysilane,(3-trimethoxysilylpropyl)diethylene-triamine,n-butylaminopropyltrimethoxy-silane,n-ethylaminoisobutyltrimethoxy-silane,methylaminopropyltrimethoxy-silane,n-phenylaminopropyltrimethoxy-silane,3-(n-allylamino)propyltrimethoxy-silane,(cyclohexylaminomethyl)tri-ethoxysilane,cyclohexylaminopropyltrimeth-oxysilane,n-ethylaminoisobutylmethyl-diethoxysilane,(phenylaminomethyl)methyl-dimethoxysilane,n-phenylaminomethyltriethoxysilane,n-methylaminopropylmethyl-dimethoxysilane,3-(n-styrylmethyl-2-aminoethylamino)-propyltrimethoxysilanehydrochloride, n-(trimethoxysilylpropyl)isothio-uronium chloride,bis┌(3-trimethoxysilyl)propyl┐-ethylenediamine,bis[(3-trimethoxysilyl)propyl]-ethylenediamine,bis[3-(triethoxysilyl)propyl]urea, bis(trimethoxysilylpropyl)urea,bis(methyldiethoxysilylpropyl)amine, ureidopropyltriethoxysilane,acetamidopropyltrimethoxysilane,n-[5-(trimethoxysilyl)-2-aza-1-oxo-pentyl]caprolactam andureidopropyltrimethoxysilane, but the present invention is not limitedthereto.

The aminosilane compound may be contained at 0.000001 to 30 parts byweight, 0.001 to 15 parts by weight, 0.01 to 5 parts by weight withrespect to 100 parts by weight of the total composition. When thecontent of the aminosilane compound is less than 0.000001 part byweight, it is difficult to exhibit an effect of continuously enhancingprotein strength, and when the content of the aminosilane compound ismore than 30 parts by weight, an amine present in the aminosilanecompound is contained in the composition at an excessively large amount,so that there may be a problem with stability of a formulation.

In the present invention, the reaction mediator includes one or moreselected from the group consisting of a carbodiimide-based compound, adihydroxyquinoline-based compound, an aminium-based compound, aphosphonium-based compound and an enzyme.

The carbodiimide-based compound is a compound having at least onemethane diimine (—N═C═N—) in the molecule, and may include a componentrepresented by Formula 4 below:

In Formula 4, A is a monomer which has one or more structures selectedfrom the group consisting of

and includes isocyanate (*) groups at both ends of a repeat unit,wherein R is each independently hydrogen; or a linear, branched orcyclic hydrocarbon having 1 to 500 carbon atoms or a benzene ringhydrocarbon, which includes one or more double bonds in a part of themolecule, or is substituted with one or more atoms selected from thegroup consisting of O, N, S, P and Si or substituted in an anionic,cationic or amphoteric form, or includes a structure to which a metalion is bonded in a salt form; and m is an integer of 1 to 100.

The carbodiimide-based compound may be one or more selected from thegroup consisting of 1,1′-methylene-bis-(4-isocyanatocyclohexane)-,homopolymer, polyethylene glycol mono-Me-ether-blocked;1,1′-methylene-bis-(3-isocyanatocyclohexane)-, homopolymer, polyethyleneglycol mono-Me-ether-blocked;1,1′-methylene-bis-(4-isocyanatocycloheptane)-, homopolymer,polyethylene glycol mono-Me-ether-blocked;1,1′-methylene-bis-(3-isocyanatocycloheptane)-, homopolymer,polyethylene glycol mono-Me-ether-blocked;1,1′-methylene-bis-(3-isocyanatocyclopentane)-, homopolymer,polyethylene glycol mono-Me-ether-blocked; benzene;1,3-bis(1-isocyanato-1-methylethyl)-, homopolymer, polyethylene glycolmono-Me-ether-blocked; 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide;and 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide HCl, but the presentinvention is not limited thereto.

In one exemplary embodiment, as a reaction mediator, acarbodiimide-based compound such as benzene;1,3-bis(1-isocyanato-1-methylethyl)-, homopolymer, polyethylene glycolmono-Me-ether-blocked; or 1,1′-methylene-bis-(4-isocyanatocyclohexane)-,homopolymer, polyethylene glycol mono-Me-ether-blocked may be used.

The dihydroxyquinoline-based compound may be a compound represented byFormula 5 below:

In Formula 5, R₁ may be one selected from the group consisting ofaqueous non-ionic polymers; bead- and resin-type polymer resins; andsilica beads, and R₂ and R₃ may be each independently a linear orbranched saturated alkyl group having 1 to 10 carbon atoms; or anunsaturated alkyl group.

The aqueous non-ionic polymer may be one or more polymers selected fromthe group consisting of polyethylene glycol (PEG), polyvinyl alcohol(PVA), polyethylene oxide (PEO), polyacrylonitrile (PAN),polyvinylpyrrolidone (PVP), polyisopropylacrylamide (PNIPPAm), acellulose derivative, a starch derivative, dextran and guar gum, eachhaving a molecular weight of less than 20,000 Da, but the presentinvention is not limited thereto. When the molecular weight of theaqueous non-ionic polymer is 20,000 Da or more, a proportion of thedihydroxyquinoline-based compound inducing covalent bonding is toosmall, it is difficult to exhibit a glossy effect.

The bead- and resin-type polymer resin may be a polymer prepared bypolymerizing one or more monomers selected from the group consisting ofstyrene, ethylene, butadiene, acrylonitrile, methylstyrene,terephthalate, ethylene chloride, ketone ether, imide ether, sulfoneether, phthalamide, phenylene ether, phenylene oxide, phenylsulfide,sulfone, urethane, vinylidene fluoride and tetrafluoroethylene, eachhaving a molecular weight of less than 20,000 Da, but the presentinvention is not limited thereto.

When the molecular weight of the bead- and resin-type polymer resins ismore than 20,000 Da, a proportion of the dihydroxyquinoline-basedcompound inducing covalent bonding is very small, it is difficult toexhibit a glossy effect.

The silica bead may have a diameter of 100 nm to 1 mm, for example, 100nm to 100 μm, 1 to 100 μm, or 1 to 70 μm. When the diameter of thesilica bead is smaller than 100 nm, there may be a problem with safetyon the skin surface, and when the diameter of the silica bead is largerthan 1 mm, there are problems with formulation and stabilization.

The reaction mediator of the present invention may be an aminium-basedcompound, which is a cationic compound having a —R₃NH⁺ structure formedby the hydrogenation of one amine; or a phosphonium salt (PH₄ ⁺) seriescompound having a PH₄ ⁺ structure as a polyatomic cation, but thepresent invention is not limited thereto. R₃ is hydrogen; or a linear,branched or cyclic hydrocarbon having 1 to 500 carbon atoms or a benzenering hydrocarbon, which includes one or more double bonds in a part ofthe molecule, or is substituted with one or more atoms selected from thegroup consisting of O, N, S, P and Si or substituted in an anionic,cationic or amphoteric form, or includes a structure to which a metalion is bonded in a salt form.

The aminium-based compound may be one or more selected from the groupconsisting ofN-[(1H-benzotriazol-1-yl)(dimethylamino)methylene]-N-methylmethanaminiumhexafluorophosphate N-oxide, N-[(1H-benzotriazol-1-yl)(dimethylamino)methylene]-N-methylmethanaminium hexafluoroborate,2-(3,4-(N-┌(1H-benzotriazol-1-yl)(dimethylamino)methylene┐-N-methylmethanaminiumhexafluorophosphate N-oxide,2-(3,4-dihydro-4-oxo-1,2,3-benzotriazin-3-yl)-1,1,3,3,-tetramethyluronium tetrafluoroborate,O-(3,4,-dihydro-4-oxo-5-azabenzo-1,2,3-triazin-3-yl)-1,1,3,3,-tetramethyluroniumhexafluoroborate, O-(3,4,-dihydro-4-oxo-5-azabenzo-1,2,3-triazin-3-yl)-1,1,3,3 tetramethyluroniumhexafluorophosphate, 2-(2-oxo-1(2H)-pyridyl-1,1,3,3,-tetramethyluroniumtetrafluoroborate, 2-(2-oxo-1(2H)-pyridyl-1,1,3,3-tetramethyluroniumtetrafluorophosphate, 2-succinimido-1,1,3,3-tetramethyluroniumtetrafluoroborate), 2-succinimido-1,1,3,3-tetramethyluroniumtetrafluorophosphate,N,N,N′,N′-bis(tetramethylene-O-pentafluorophenyluroniumtetrafluoroborate,N,N,N′,N′-bis(tetramethylene-O-pentafluorophenyluroniumhexafluorophosphate,N-[6-trifluoromethyl(1H-benzotriazol-1-yl)(dimethylamino)methylene]-N-methylmethanaminium tetrafluoroborate N-oxide,N-[6-trifluoromethyl(1H-benzotriazol-1-yl)(dimethylamino)methylenel-N-methylmethanaminium hexafluorophosphateN-oxide,N-[(dimethylamino)-1H-1,2,3-triazolo[4,5,b]pyridin-1-yl]methylene]-N-methylmethanaminiumhexafluorophosphate N-oxide,N-[(dimethylamino)-1H-1,2,3-triazolo[4,5-b]pyridin-1-ylmethylene]-N-methylmethanaminium tetrafluoroborate N-oxide,N-[(dimethylamino)-1H-1,2,3-triazolo[4,5-b]pyridin-1-ylmethylene]-N-methylmethanaminiumhexafluorophosphate N-sulfide,S-(1-oxido-1-pyridinyl)-1,1,3,3-tetramethylthiouroniumhexafluorophosphate,O-[cyano(ethoxycarbonyl)methyleneamino]-N,N,N′,N′-tetramethyluroniumtetrafluoroborate, O-[cyano(ethoxycarbonyl) methyleneamino]-N,N,N′,N′-tetramethyluronium hexafluorophosphate, O-[(dicyanomethylidene)-amino]-1,1,3,3-tetramethyluronium hexafluorophosphate,O-[(dimethoxycarbonylmethylidene)-amino]-1,1,3,3-tetramethyluroniumhexafluorophosphate, N-[(cyano(pyridin-2-yl)methylene aminooxy)(dimethylamino)methylenel-N-methylmethanaminium hexafluorophosphate,2-(5-norbornene-2,3-dicarboximido)-1,1,3,3-tetramethyluroniumtetrafluoroborate, 2-phthalimido-1,1,3,3-tetramethyluroniumtetrafluoroborate, bis(tetra methylene)chloroformamidiniumhexafluorophosphate, (1H-benzotriazol-1-yl)(1-pyrollidinylmethylene)pyrrolidinium hexafluorophosphate N-oxide,1-(1-pyrrolidinyl-1H-1,2,3-triazolo[4,5-b]pyridin-1-ylmethylene)pyrrolidinium hexafluorophosphate N-oxide,O-(3,4-dihydro-4-oxo-1,2,3-benzotriazin-3-yl)-1,1,3,3-bis(tetramethylene)uranium hexafluorophosphate,O-(3,4-dihydro-4-oxo-5-azabenzo-1,2,3-triazin-3-yl)-1,1,3,3-bis(tetramethylene)uranium hexafluorophosphate, N,N,N′,N′-bis(tetramethylene)-O-pentafluoro phenyluronium hexafluorphosphate,N,N,N′,N′-bis(tetramethylene)-S-pentafluorothiophenyluroniumhexafluorphosphate,1-(1-pyrrolidinyl-1H-1,2,3-triazolo[4,5-b]pyridin-1-ylmethylene)pyrrolidinium hexafluorophosphate N-sulfide,N,N,N′,N′-bis(tetramethylene)-O-2-nitrophenyluroniumhexafluorophosphate,N,N,N′,N′-bis(tetramethylene)-O-pentafluorophenyluroniumhexafluorohosphate, O-(benzotriazol-1-yl)-1,1,3,3-bis(pentamethylene)hexafluorophosphate,O-(7-azabenzotriazol-1-yl)-1,1,3,3-bis(pentamethylene)-uroniumhexafluorophosphate, 2-[2-oxo-1(2H)-pyridyl]-1,1,3,3-bis(pentamethylene)uronium tetrafluoroborate, 2-chloro-1,3-dimethylimidazolidiniumhexafluorophosphate, O-(benzotriazol-1-yl)-1,3-dimethyl-1,3-dimethyleneuronium hexafluorophosphate, O-(7-azabenzotriazol-1-yl)-1,3-dimethyl-1,3-dimethyleneuronium hexafluorophosphate,2-chloro-1,3-dimethylpyrimidinium hexafluorophosphate,O-(benzotriazol-1-yl)-1,3-dimethyl-1,3-trimethyleneuroniumhexafluorophosphate,O-(7-azabenzotriazol-1-yl)-1,3-dimethyl-1,3-trimethyleneuroniumhexafluorophosphate, (7-benzotriazol-yl)-1,1,3-trimethyl-1-hexafluorophosphate,(7-azabenzotriazol-yl)-1,1,3-trimethyl-1-phenyluroniumhexafluorophosphate,O-(1H-benzotriazol-1-yl)-1,1-dimethyl-3,3-tetramethylene uraniumhexafluorophosphate,O-(1H-1,2,3-triazolo[4,5-b]pyridin-yl)-1,1-dimethyl-3,3-tetramethyleneuroniumhexafluorophosphate,O-(1H-benzotriazol-1-yl)-1,1-dimethyl-3,3-pentamethylene uraniumhexafluorophosphate,6-chloro-1((dimethylamino)(morpholino)methylene)-1H-benzo triazoliumhexafluorophosphate-3oxide,3-((dimethylamino)-(morpholino)methylene)-1H-[1,2,3]triazolo[4,5-b]pyridiniumhexafluorophosphate,6-trifluoromethyl-1-((dimethylamino)-(morpholino)methylene)-1H-benzotriazoliumhexafluorophosphate-3-oxide,1-((dimethylamino)-(morpholino)) oxypentafluorophenylmetheniminiumhexafluorophosphate,1-[(1-(cyano-2-ethoxy-2-oxoethylideneaminooxy)-dimethylaminomorpholinomethylene)]methanaminium hexafluorophosphate,1-[(1-(dicyanomethyleneaminooxy) dimethylaminomorpholinomethylene)]methanaminium hexafluorophosphate,1-[(1,3-diethoxy-1,3-dioxopropan-2-ylideneaminooxy)dimethylaminomorpholinomethylene)]methanaminium hexafluorophosphate,N-[(cyano(pyridin-2-yl)methyleneaminooxy)(dimethylamino)methylene]-N-morpholinomethanaminiumhexafluorophosphate,1-[(1-(cyano-2-ethoxy-2-oxoethylideneaminooxy)dimethylaminopyrrolodinomethylene)]methanaminium hexafluorophosphate,1-((dicyanomethyleneaminooxy) morpholinomethylene)pyrrolidiniumhexafluorophosphate,1-[(1,3-diethoxy-1,3-dioxopropan-2-yldeneaminooxy)-dimethylamino-pyrrolodinomethylene]]methanaminiumhexafluorophosphate, 1-[(1-(cyano-2-ethoxy-2-oxoethylideneaminooxy)-dimethylamino-pyrrolodinomethylene)]methanaminiumhexafluororphosphate,1-((1-cyano-2-ethoxy-2-oxoethylideneaminooxy)(morpholino)methylene)pyrrolidinium hexafluorophosphate,benzotriazol-1-yloxy-N,N-dimethyl-methanaminium hexachloroantimonate,5-(1 H-benzotriazol-1-yloxy)-3,4-dihydro-1-methyl-2H-pyrroliumhexachloroantimonate,5-(7-azabenzotriazol-1-yloxy)-3,4-dihydro-1-methyl-2H-pyrroliumhexachloroantimonate, 1-(1H-benzotriaol-1-yloxy)phenyl-methylenepyrrolidinium hexachloroantimonate,5-(pentafluorophenyloxy)-3,4-dihydro-1-methyl-2H-pyrroliumhexachloroantimonite,5-(succinimidyloxy)-3,4-dihydro-1-methyl-2H-pyrroliumhexachloroantimonate,5-(3′,4′-dihydro-4′-oxo-1′,2′,3′-benzotriazin-3′-yloxy)-3,4-diydro-1-methyl-2H-pyrroliumhexachloroantimonate) and a derivative thereof, but the presentinvention is not limited thereto.

The phosphonium-based compound may be one or more selected from thegroup consisting of benzotriazol-1-yloxytris(dimethylamino) phosphoniumhexafluorophosphate, bromotris (dimethylamino) phosphoniumhexafluorophosphate, chlorotri (pyrrolidino) phosphoniumhexafluorophosphate, bromotri (pyrrolidino) phosphoniumhexafluorophosphate, chloro-tris(dimethylamino) phosphoniumhexafluorophosphate, benzotriazol-1-yloxytri (pyrrolidino) phosphoniumhexafluorophosphate, └(7-azabenzotriazol-1-yl)oxy┘tris-(dimethyl amino)phosphonium hexafluorophosphate,[(7-azabenzotriazol-1-yl)oxy]tris-(pyrrolidino) phosphoniumhexaflurophosphate,O-[(cyano-(ethoxycarbonyl)methyliden)-amino]-yloxytripyrrolidinophosphonium hexafluorophosphate,[(6-nitrobenzotriazol-yl)oxyltris-(pyrrolidino) phosphoniumhexafluorophosphate,[(6-trifluoromethyl)benzotriazol-1-yl]oxy-tris(pyrrolidino)phosphoniumhexafluorophosphate,[4-nitro-6-(trifluoromethyl)benzotriazol-1-yl]oxyl]tris(pyrrolidino)phosphonium hexafluorophosphate, (6-chloro-benzotriazol-1-yloxy)tris(pyrrolidino)-phosphonium hexafluorophosphate,N′,N′,N′,N′-bis(tetramethylene)-O-pentafluoro phenyluroniumhexafluorophosphate, (pyridyl-2-thio)tris (pyrrolidino)-phosphoniumhexafluorophosphate,[(3,4-dihydro-4-oxo-1,2,3-benzotriazin-3-yl)oxy]tris-(pyrrolidino)phosphoniumhexafluorophosphate,[(3,4-dihydro-4-oxo-5-azabenzo-1,2,3-triazin-3-yl]tris-(pyrrolidino)phosphonium hexafluorophosphate and a derivative thereof, but thepresent invention is not limited thereto.

The enzyme may be transglutaminase, sortase A, tyrosinase,laccase/peroxidase or lysyl oxidase/amine oxidase, which mediatesbioconjugation between the amine of glutamine and the amine of lysine,but the present invention is not limited thereto.

The reaction mediator may be used at 0.000001 to 10 parts by weight,0.001 to 7 parts by weight, or 0.01 to 5 parts by weight with respect to100 parts by weight of the total composition. When the content of thereaction mediator is less than 0.000001 part by weight, it is difficultto exhibit a continuous surface improvement effect, and when the contentof the reaction mediator is more than 10 parts by weight, the reactionmediator excessively present in an amount higher than a reactionposition present in a substrate consisting of a protein reacts with afunctional component while not reacting with the protein substrate, andthus it does not help to improve the sustainability of glossiness, andis rather lost.

This reaction may be performed under a condition of pH 2 to pH 12, andmore preferably, at pH 3 to pH 10, and most preferably, in a weaklyacidic aqueous solution of pH 4.5 to pH 8 to maximize reactionefficiency. The reaction is finished within 1 minute to 30 minutes.

The composition may be used by coating, spraying, dilution, or othersimilar methods, and dehydration may be promoted under a mild conditionsuch as an elevated temperature (20 to 80° C.) at which thermaldenaturation of a protein does not occur, and thus the reactionefficiency may be enhanced.

In one exemplary embodiment, using a carbodiimide-based compound as areaction mediator, a reaction between a carboxyl group on the surface ofthe substrate consisting of a protein and an amine group oftriethoxyaminopropylsilane is illustrated in Reaction Scheme 1 below.

As shown in Reaction Scheme 1, an amino acid linked to hair, scalp,skin, finger/toe nails, leather and/or fiber is not easily detached ingeneral cleansing with a shampoo, a detergent or a soap, and thus thestructure attached to the hair, scalp, skin, finger/toe nails, leatherand/or fiber may be almost permanently maintained.

The composition for enhancing protein strength according to the presentinvention may further include a component capable of improving a proteinstrength enhancement effect, other than the aminosilane compound and thereaction mediator. For example, the composition may be easily preparedwhen mixed with a fatty acid such as palmitic acid, stearic acid, lauricacid or myristic acid, a cationic surfactant such as a fatty alcohol, ora linear or branched long-chain alkyl quaternary ammonium salt, acationic polymer such as cationic cellulose, cationic guar gum orcationic polyvinylpyrrolidone, or silicone. In addition, for formulationas a cosmetic preparation, components for preparing a cosmetic such as asolvent, a surfactant, a thickening agent, a stabilizer, a preservative,a coloring agent, a pH adjustor, a sequestering agent, a coloring agent,a pearlizing agent, an appearance improving agent, a pigment and powderparticles may be further included. The components for preparation may beused at 40 to 99 parts by weight with respect to 100 parts by weight ofthe total composition.

The composition for enhancing protein strength according to the presentinvention may include an aminosilane compound with a functional groupand thus is able to be used in the formulation of a composition forenhancing protein strength. Examples of the composition for hair mayinclude all cosmetic preparations capable of being used for hair, forexample, a pre-shampoo product, a shampoo, a rinse, a treatment, a wax,a spray, a mousse, a hair lotion, an essence, a hair cream, a pack, amask, a tablet, a patch, a strip, a slave, a permanent hair dye, atemporary hair dye and a hair waving agent, examples of the compositionfor skin may include all cosmetic preparations capable of being used forskin, for example, a skin toner, a lotion, an essence, a serum, a cream,a gel, a foundation, a powder, a makeup base, a point makeup product, amask and a patch, examples of the composition for fabric may include allfabric care preparations capable of being used in fabric, for example, afabric softener, a fabric dye, a washing detergent, a treatment, apre/after care agent, a laundry supplement, a spot stain remover and aspray. Examples of the composition for leather may include allpreparations for treating leather, for example, a cream, a lotion, anessence, a serum, a gel, a wax, a spray, a cleanser, a cleaner, a spotstain remover, a salve, a temporary dye, a permanent dye, a polish, astripping agent and a sheet, but the present invention is not limitedthereto.

To enhance the protein strength enhancement effect in the compositionfor enhancing protein strength according to the present invention, adi-basic acid ester oil such as dioctyl succinate, dioctyl adipate ordiethyl sebacate, polyol, polyethylene glycol, propylene glycol,hexylene glycol, butanediol and their isomers, and glycerol, benzylalcohol, ethoxydiglycol and their derivatives may be used. Theabove-mentioned solvents increase the permeability of hair and skin andare used as solvents for a poorly soluble substance.

The advantages and features of the present invention, and methods ofattaining the same will be clarified with reference to examples thatwill be described below in detail. However, the present invention may beembodied in a variety of different forms and is not limited to theexamples described below, and the examples are merely provided tocomplete the disclosure of the present invention, and fully inform thescope of the present invention to those of ordinary skill in the art.

EXAMPLES 1 TO 12 AND COMPARATIVE EXAMPLES 1 TO 4

Shampoo compositions for enhancing the strength of a hair proteinaccording to Examples 1 to 12 and Comparative Examples 1 to 4 wereprepared with compositions and contents shown in Tables 1 and 2 below.Hair was thoroughly washed with each of the prepared shampoocompositions, and then about 3 g of a towel-dried hair tress wasprepared, and therefrom, 50 strings of the hair were randomly extractedto measure a tensile strength using a hair tensile strength tester, andthe result was determined to be hair tensile strength before use of thecomposition. Shampooing was performed 30 times on the specimens preparedfrom the same tress using 0.3 g of each composition, and then subjectedto the measurement of tensile strength to evaluate strength variations.The results obtained from the all compositions were compared.

TABLE 1 Example Example Example Example Example Example Example ExampleExample Example Example Example (Parts by weight) 1 2 3 4 5 6 7 8 9 1011 12 Water To To To To To To To To To To To To 100 100 100 100 100 100100 100 100 100 100 100 Polyquaternium-10 0.5 0.5 0.5 0.5 0.5 0.5 0.50.5 0.5 0.5 0.5 0.5 EDTA 4Na 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.050.05 0.05 0.05 0.05 Sodium Lauryl 35 35 35 35 35 35 35 35 35 35 35 35Ether(2 mole) Sulfate (30%) Cocamidopropyl 15 15 15 15 15 15 15 15 15 1515 15 Betaine (30%) Fragrance 0.9 0.9 0.9 0.9 0.9 0.9 0.9 0.9 0.9 0.90.9 0.9 3- 0.5 — — — 0.5 — — — 0.2 0.2 0.5 0.5aminopropyltriethoxysilane 3- 0.5 — — — 0.5 — — — 0.2 0.2 — —aminopropylmethyldiethoxy- silane 3- — 0.5 — — — 0.5 — — 0.2 0.2 — —aminopropyldimethylethoxy- silane p- — — — 0.5 — — — 0.5 0.2 0.2 — —aminophenyltriethoxysilane Benzene, 1,3-bis(1- 1 1 1 1 — — — — — 0.5 — —isocyanato-1-methylethyl)-, homopolymer, polyethylene glycolmono-Me-ether- blocked 1,1′- methylene-bis-(4- — — — — 1 1 1 1 1 0.5 — —isocyanatocyclohexane)-, homopolymer, polyethylene glycolmono-Me-ether-blocked N-[(1H-benzotriazole-1- — — — — — — — — — — 1 —yl)(dimethylamino) methylene]-N- methylmethaneaminiumhexafluorophosphate N-oxide Benzotriazole-1- — — — — — — — — — — — 1yloxytris(dimethylamino) phosphonium hexafluorophosphate pH adjustorq.s. q.s. q.s. q.s. q.s. q.s. q.s. q.s. q.s. q.s. q.s. q.s. Total 100100 100 100 100 100 100 100 100 100 100 100

TABLE 2 (Parts by weight) Comparative Comparative ComparativeComparative Example 1 Example 2 Example 3 Example 4 Water To 100 To 100To 100 To 100 Polyquaternium-10 0.5 0.5 0.5 0.5 EDTA 4Na 0.05 0.05 0.050.05 Sodium Lauryl Ether(2 mole) Sulfate (30%) 35 35 35 35Cocamidopropyl Betaine (30%) 15 15 15 15 Fragrance 0.9 0.9 0.9 0.93-aminopropyl triethoxysilane 0.5 — — —3-aminopropylmethyldiethoxysilane — 0.5 — —3-aminopropyldimethylethoxysilane — — 0.5 — p-aminophenyltriethoxysilane— — — 0.5 Benzene, 1,3-bis(1-isocyanato-1- — — — — methylethyl)-,homopolymer, polyethylene glycol mono-Me-ether-blocked1,1′-methylene-bis-(4- — — — — isocyanatocyclohexane)-, homopolymer,polyethylene glycol mono-Me-ether-blocked N-[(1H-benzotriazole-1- — — —— yl)(dimethylamino)methylene]-N- methylmethaneaminiumhexafluorophosphate N-oxide Benzotriazole-1-yloxytris(dimethylamino) — —— — phosphonium hexafluorophosphate pH adjustor q.s. q.s. q.s. q.s.Total 100 100 100 100

TABLE 3 Example Example Example Example Example Example Example ExampleExample Example Example Example 1 2 3 4 5 6 7 8 9 10 11 12 Tensile 56.355.2 53.3 52.9 76.3 75.2 73.4 73 75.8 76.9 55.2 53.1 strength increaserate (%)

TABLE 4 Comparative Comparative Comparative Comparative Example 1Example 2 Example 3 Example 4 Tensile 31.6 28.3 25.6 20.3 strengthIncrease rate (%)

As shown in Tables 3 and 4, the experiments for the compositions ofExamples 1 to 12 and Comparative Examples 1 to 4 showed that, comparedto the compositions of Comparative Examples 1 to 4 including only anaminosilane component without a reaction mediator, the compositionsincluding, as a reaction mediator, benzene,1,3-bis(1-isocyanato-1-methylethyl)-, homopolymer, polyethylene glycolmono-Me-ether-blocked (Examples 1 to 4 and 10) or1,1′-methylene-bis-(4-isocyanatocyclohexane)-, homopolymer, polyethyleneglycol mono-Me-ether-blocked (Examples 5 to 10),N-[(1H-benzotriazole-1-yl)(dimethylamino)methylene]-N-methylmethaneaminium hexafluorophosphateN-oxide (Example 11) andbenzotriazole-1-yloxytris(dimethylamino)phosphonium hexafluorophosphate(Example 12) exhibit a very excellent hair tensile strength enhancementeffect after the use of the shampoos.

EXAMPLES 13 TO 24 AND COMPARATIVE EXAMPLES 5 TO 8

Body wash compositions for enhancing elasticity through skinstrengthening according to Examples 13 to 24 and Comparative Examples 5to 8 were prepared with compositions and contents shown in Tables 5 and6. The initial elasticity of artificial skin was measured, theelasticity of artificial skin after 30 times of use was assessed usingeach composition prepared previously, and the skin elasticityenhancement effect of the compositions were compared.

TABLE 5 Example Example Example Example Example Example Example ExampleExample Example Example Example (Parts by weight) 13 14 15 16 17 18 1920 21 22 23 24 Water To To To To To To To To To To To To 100 100 100 100100 100 100 100 100 100 100 100 Polyquaternium-7 0.5 0.5 0.5 0.5 0.5 0.50.5 0.5 0.5 0.5 0.5 0.5 EDTA 4Na 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.050.05 0.05 0.05 0.05 Lauric Acid 3 3 3 3 3 3 3 3 3 3 3 3 Myristic acid 44 4 4 4 4 4 4 4 4 4 4 Sodium Lauryl Ether 20 20 20 20 20 20 20 20 20 2020 20 (2 mole) Sulfate (30%) Cocamidopropyl 15 15 15 15 15 15 15 15 1515 15 15 Betaine (30%) Fragrance 0.9 0.9 0.9 0.9 0.9 0.9 0.9 0.9 0.9 0.90.9 0.9 3- 0.5 — — — 0.5 — — — 0.2 0.2 0.5 0.5aminopropyltriethoxysilane 3- — 0.5 — — — 0.5 — — 0.2 0.2 — —aminopropylmethyldiethoxy- silane 3- — — 0.5 — — — 0.5 — 0.2 0.2 — —aminopropyldimethylethoxy- silane p- — — — 0.5 — — — 0.5 0.2 0.2 — —aminophenyltriethoxysilane Benzene, 1,3-bis(1- 1 1 1 1 — — — — — 0.5 — —isocyanato-1-methylethyl)-, homopolymer, polyethylene glycolmono-Me-ether-blocked 1,1′- methylene-bis-(4- — — — — 1 1 1 1 1 0.5 — —isocyanatocyclohexane)-, homopolymer, polyethylene glycolmono-Me-ether-blocked N-[(1H-benzotriazole-1- yl)(dimethylamino)methylene]-N- — — — — — — — — — — 1 — methylmethaneaminiumhexafluorophosphate N-oxide Benzotriazole-1- — — — — — — — — — — — 1yloxytris(dimethylamino) phosphonium hexafluorophosphate pH adjustorq.s. q.s. q.s. q.s. q.s. q.s. q.s. q.s. q.s. q.s. q.s. q.s. Total 100100 100 100 100 100 100 100 100 100 100 100

TABLE 6 (Parts by weight) Comparative Comparative ComparativeComparative Example 5 Example 6 Example 7 Example 8 Water To 100 To 100To 100 To 100 Polyquaternium-7 0.5 0.5 0.5 0.5 EDTA 4Na 0.05 0.05 0.050.05 Lauric Acid 3 3 3 3 Myristic acid 4 4 4 4 Sodium Lauryl Ether (2mole) Sulfate (30%) 20 20 20 20 Cocamidopropyl Betaine (30%) 15 15 15 15Fragrance 0.9 0.9 0.9 0.9 3-aminopropyltriethoxysilane 0.5 — — —3-aminopropylmethyldiethoxysilane — 0.5 — —3-aminopropyldimethylethoxysilane — — 0.5 — p-aminophenyltriethoxysilane— — — 0.5 Benzene, 1,3-bis(1-isocyanato-1- — — — — methylethyl)-,homopolymer, polyethylene glycol mono-Me-ether-blocked1,1′-methylene-bis-(4- — — — — isocyanatocyclohexane)-, homopolymer,polyethylene glycol mono-Me-ether-blocked N-[(1H-benzotriazole-1- — — —— yl)(dimethylamino)methylene]-N- methylmethaneaminiumhexafluorophosphate N-oxide Benzotriazole-1-yloxytris(dimethylamino) — —— — phosphonium hexafluorophosphate pH adjustor q.s. q.s. q.s. q.s.Total 100 100 100 100

TABLE 7 Example Example Example Example Example Example Example ExampleExample Example Example Example 13 14 15 16 17 18 19 20 21 22 23 24 Skin23.2 22.1 22 20.9 23.1 22.3 22.1 20 33.4 34.7 21.7 22.2 elasticityincrease rate (%)

TABLE 8 Comparative Comparative Comparative Comparative Example 5Example 6 Example 7 Example 8 Skin elasticity 10.2 9.8 9.7 8.5 increaserate (%)

As shown in Tables 7 and 8, the experiments for the compositionsaccording to Examples 13 to 24 and Comparative Examples 5 to 8 showedthat, compared to the compositions of Comparative Examples 5 to 8including only an amino acid component without a reaction mediator, thecompositions including, as a reaction mediator, benzene,1,3-bis(1-isocyanato-1-methylethyl)-, homopolymer, polyethylene glycolmono-Me-ether-blocked (Examples 13 to 16 and 22) or1,1′-methylene-bis-(4-isocyanatocyclohexane)-, homopolymer, polyethyleneglycol mono-Me-ether-blocked (Examples 17 to 22),N-[(1H-benzotriazole-1-yl)(dimethylamino)methylene]-N-methylmethaneaminium hexafluorophosphateN-oxide (Example 23) andbenzotriazole-1-yloxytris(dimethylamino)phosphonium hexafluorophosphate(Example 24) exhibit a very excellent skin elasticity improvement effectafter the use of the body washes.

EXAMPLES 25 TO 36 AND COMPARATIVE EXAMPLES 9 TO 12

Compositions for strengthening nails according to Examples 25 to 36 andComparative Examples 9 to 12 were prepared with compositions andcontents shown in Tables 9 and 10 below. Each composition was used on 10consumers for 20 days, and then conditions of nails such as splitting orcracking were compared to those of the initial conditions of the nails,and sensory evaluated on a 5-point scale (5: very good, 4: good, 3: nodifference, 2: almost no effect, 1: no effect at all) for comparison.

TABLE 9 Example Example Example Example Example Example Example ExampleExample Example Example Example (Parts by weight) 25 26 27 28 29 30 3132 33 34 35 36 Water To To To To To To To To To To To To 100 100 100 100100 100 100 100 100 100 100 100 Amodimethicone 0.5 0.5 0.5 0.5 0.5 0.50.5 0.5 0.5 0.5 0.5 0.5 18-Methyl Eicosanoic 1 1 1 1 1 1 1 1 1 1 1 1Acid Isostearylamine 1 1 1 1 1 1 1 1 1 1 1 1 Glycerin 2 2 2 2 2 2 2 2 22 2 2 Butylene glycol 2 2 2 2 2 2 2 2 2 2 2 2 Propylene glycol 2 2 2 2 22 2 2 2 2 2 2 Fragrance 0.9 0.9 0.9 0.9 0.9 0.9 0.9 0.9 0.9 0.9 0.9 0.9Carboxyvinyl polymer 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1Polysolvate 60 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 Liquidparaffin 5 5 5 5 5 5 5 5 5 5 5 5 Caprylic/capric 2.5 2.5 2.5 2.5 2.5 2.52.5 2.5 2.5 2.5 2.5 2.5 triglyceride Squalane 2.5 2.5 2.5 2.5 2.5 2.52.5 2.5 2.5 2.5 2.5 2.5 Cetearyl glucoside 2 2 2 2 2 2 2 2 2 2 2 2Triethanolamine 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 3- 0.5 —— — 0.5 — — — 0.2 0.2 0.5 0.5 aminopropyltriethoxysilane 3- — 0.5 — — —0.5 — — 0.2 0.2 — — aminopropylmethyldiethoxy- silane 3- — — 0.5 — — —0.5 — 0.2 0.2 — — aminopropyldimethylethoxy- silane p- — — — 0.5 — — —0.5 0.2 0.2 — — aminophenyltriethoxysilane Benzene, 1,3-bis(1- 1 1 1 1 —— — — — 0.5 — — isocyanato-1- methylethyl)-, homopolymer, polyethyleneglycol mono-Me-ether-blocked 1,1′-methylene-bis-(4- — — — — 1 1 1 1 10.5 — — isocyanatocyclohexane)-, homopolymer, polyethylene glycolmono-Me-ether-blocked N-[(1H-benzotriazole-1- — — — — — — — — — — 1 —yl)(dimethylamino) methylene]-N- methylmethaneaminiumhexafluorophosphate N-oxide Benzotriazole-1- — — — — — — — — — — — 1yloxytris(dimethylamino) phosphonium hexafluorophosphate pH adjustorq.s. q.s. q.s. q.s. q.s. q.s. q.s. q.s. q.s. q.s. q.s. q.s. Total 100100 100 100 100 100 100 100 100 100 100 100

TABLE 10 (Parts by weight) Comparative Comparative ComparativeComparative Example 9 Example 10 Example 11 Example 12 Water To 100 To100 To 100 To 100 Amodimethicone 0.5 0.5 0.5 0.5 18-Methyl EicosanoicAcid 1 1 1 1 Isostearylamine 1 1 1 1 Glycerin 2 2 2 2 Butylene glycol 22 2 2 Propylene glycol 2 2 2 2 Fragrance 0.9 0.9 0.9 0.9 Carboxyvinylpolymer 0.1 0.1 0.1 0.1 Polysolvate 60 1.5 1.5 1.5 1.5 Liquid paraffin 55 5 5 Caprylic/caprictriglyceride 2.5 2.5 2.5 2.5 Squalane 2.5 2.5 2.52.5 Cetearyl glucoside 2 2 2 2 triethanolamine 0.1 0.1 0.1 0.13-aminopropyltriethoxysilane 0.5 — — — 3-aminopropylmethyldiethoxysilane— 0.5 — — 3-aminopropyldimethylethoxysilane — — 0.5 —p-aminophenyltriethoxysilane — — — 0.5 Benzene, 1,3-bis(1-isocyanato-1-— — — — methylethyl)-, homopolymer, polyethylene glycolmono-Me-ether-blocked 1,1′-methylene-bis-(4- — — — —isocyanatocyclohexane)-, homopolymer, polyethylene glycolmono-Me-ether-blocked N-[(1H-benzotriazole-1- — — — —yl)(dimethylamino)methylene]-N- methylmethaneaminium hexafluorophosphateN-oxide Benzotriazole-1-yloxytris(dimethylamino) — — — — phosphoniumhexafluorophosphate pH adjustor q.s. q.s. q.s. q.s. Total 100 100 100100

TABLE 11 Example Example Example Example Example Example Example ExampleExample Example Example Example 25 26 27 28 29 30 31 32 33 34 35 36Satisfaction 3.7 3.7 3.6 3.8 4.1 4 4 3.9 4.2 4.4 3.6 3.6 of nailstrength improvement effect (5-point scale)

TABLE 12 Comparative Comparative Comparative Comparative Example 9Example 10 Example 11 Example 12 Satisfaction of 2.2 2.4 2.2 2.1 nailstrength improvement effect (5-point scale)

As shown in Tables 11 and 12, experiments for the compositions accordingto Examples 25 to 36 and Comparative Examples 9 to 12 showed that,compared to the compositions of Comparative Examples 9 to 12 includingonly an amino acid component without a reaction mediator, thecompositions including, as a reaction mediator, benzene,1,3-bis(1-isocyanato-1-methylethyl)-, homopolymer, polyethylene glycolmono-Me-ether-blocked (Examples 25 to 28 and 34) or1,1′-methylene-bis-(4-isocyanatocyclohexane)-, homopolymer, polyethyleneglycol mono-Me-ether-blocked (Examples 29 to 34),N-[(1H-benzotriazole-1-yl)(dimethylamino)methylene]-N-methylmethaneaminium hexafluorophosphateN-oxide (Example 35), andbenzotriazole-1-yloxytris(dimethylamino)phosphonium hexafluorophosphate(Example 36) exhibit a very excellent nail strength enhancement effect.

1. A composition for enhancing protein strength, comprising: anaminosilane compound; and a reaction mediator.
 2. The compositionaccording to claim 1, wherein the aminosilane compound is selected fromthe group consisting of the compounds represented by Formulas 1 to 3below:

wherein in Formulas 1 to 3, R₁ is each independently hydrogen; or alinear, branched or cyclic hydrocarbon having 1 to 500 carbon atoms or abenzene ring hydrocarbon, which includes one or more double bonds in apart of the molecule, or is substituted with one or more atoms selectedfrom the group consisting of O, N, S, P and Si or substituted in ananionic, cationic or amphoteric form, or includes a structure to which ametal ion is bonded in a salt form; and R₂ is each independently alinear, branched or cyclic hydrocarbon having 1 to 500 carbon atoms or abenzene ring hydrocarbon, which includes one or more double bonds in apart of the molecule, or is substituted with one or more atoms selectedfrom the group consisting of O, N, S, P and Si or substituted in ananionic, cationic or amphoteric form, or includes a structure to which ametal ion is bonded in a salt form and in which at least one primary orsecondary amine is included at an end of the molecular structure.
 3. Thecomposition according to claim 1, wherein the aminosilane compound isone or more selected from the group consisting of3-aminopropyltriethoxysilane, bis[(3-triethoxysilyl)propyl]amine,3-aminopropyltrimethoxysilane, 4-aminobutyltriethoxysilane,bis[(3-trimethoxysilyl)propyl]amine, 3-aminopropylmethyldiethoxysilane,3-aminopropyldimethylethoxysilane, 3-aminopropylmethyldimethoxysilane,aminoethylaminopropyltrimethoxysilane,aminoethylaminopropyltriethoxysilane,aminoethylaminopropylmethyldimethoxysilane,diethylenetriaminopropylmethyldimethoxysilane,piperazinylpropylmethyldimethoxysilane,(n-phenylamino)methyltrimethoxysilane,(n-phenylamino)methyltriethoxysilane,3-(n-phenylamino)propyltrimethoxysilane,n-(n-butyl)-3-aminopropyltrimethoxysilane, 4-aminobutyltriethoxysilane,m-aminophenyltrimethoxysilane, p-aminophenyltrimethoxysilane,aminophenyltrimethoxysilane, m-aminophenyltriethoxysilane,p-aminophenyltriethoxysilane, aminophenyltriethoxysilane,3-aminopropyltris(methoxyethoxy-ethoxy)silane,11-aminoundecyltriethoxysilane,3-(m-aminophenoxy(propyltrimethoxy-silane), aminopropylsilanetriol,3-aminopropylmethyldiethoxysilane,3-aminopropyldiisopropylethoxy-silane,3-aminopropyldimethylethoxysilane,n-(2-aminoethyl)-3-aminopropyltri-methoxysilane,n-(2-aminoethyl)-3-aminopropyltri-ethoxysilane,n-(6-aminohexyl)aminomethyl-triethoxysilane,n-(6-aminohexyl)aminopropyl-trimethoxysilane,n-(2-aminoethyl)-11-aminoundecyl-trimethoxysilane,(aminoethylaminomethyl)phenethyl-trimethoxysilane,n-3-[(amino(polypropylenoxy)]amino-propyltrimethoxysilane,n-(2-aminoethyl)-3-aminopropyl-silanetriol,n-(2-aminoethyl)-3-aminopropylmethyl-dimethoxysilane,n-(2-aminoethyl)-3-aminoisobutyl-methyldimethoxysilane,(aminoethylamino)-3-isobutyldi-methylmethoxysilane,(3-trimethoxysilylpropyl)diethylene-triamine,n-butylaminopropyltrimethoxy-silane,n-ethylaminoisobutyltrimethoxy-silane,n-methylaminopropyltrimethoxy-silane,n-phenylaminopropyltrimethoxy-silane,3-(n-allylamino)propyltrimethoxy-silane,(cyclohexylaminomethyl)tri-ethoxysilane,n-cyclohexylaminopropyltrimeth-oxysilane,n-ethylaminoisobutylmethyl-diethoxysilane,(phenylaminomethyl)methyl-dimethoxysilane,n-phenylaminomethyltriethoxysilane,n-methylaminopropylmethyl-dimethoxysilane,3-(n-styrylmethyl-2-aminoethylamino)-propyltrimethoxysilanehydrochloride, n-(trimethoxysilylpropyl)isothio-uronium chloride,bis[(3-trimethoxysilyl)propyl]-ethylenediamine,bis[(3-trimethoxysilyl)propyl]-ethylenediamine,bis[3-(triethoxysilyl)propyl]urea, bis(trimethoxysilylpropyl)urea,bis(methyldiethoxysilylpropyl)amine, ureidopropyltriethoxysilane,acetamidopropyltrimethoxysilane,n-[5-(trimethoxysilyl)-2-aza-1-oxo-pentyl]caprolactam andureidopropyltrimethoxysilane.
 4. The composition according to claim 1,wherein the reaction mediator is one or more selected from the groupconsisting of a carbodiimide-based compound, a dihydroxyquinoline-basedcompound, an aminium-based compound, a phosphonium-based compound and anenzyme.
 5. The composition according to claim 4, wherein thecarbodiimidie-based compound is a compound having at least one methanediimine (—N═C═N—) in the molecule.
 6. The composition according to claim4, wherein the carbodiimidie-based compound is a compound represented byFormula 4 below:

wherein in Formula 4, A is a monomer which has one or more structuresselected from the group consisting of

and includes isocyanate (*) groups at both ends of a repeat unit,wherein R is each independently hydrogen; or a linear, branched orcyclic hydrocarbon having 1 to 500 carbon atoms or a benzene ringhydrocarbon, which includes one or more double bonds in a part of themolecule, or is substituted with one or more atoms selected from thegroup consisting of O, N, S, P and Si or substituted in an anionic,cationic or amphoteric form, or includes a structure to which a metalion is bonded in a salt form; and m is an integer of 1 to
 100. 7. Thecomposition according to claim 4, wherein the dihydroxyquinoline-basedcompound is a compound represented by Formula 5 below:

Wherein in Formula 5, R₁ is one selected from the group consisting ofaqueous non-ionic polymers; bead- and resin-type polymer resins; andsilica beads, and R₂ and R₃ are each independently a linear or branchedsaturated alkyl group having 1 to 10 carbon atoms; or an unsaturatedalkyl group.
 8. The composition according to claim 7, wherein theaqueous non-ionic polymer is one or more polymers selected from thegroup consisting of polyethylene glycol (PEG), polyvinyl alcohol (PVA),polyethylene oxide (PEO), polyacrylonitrile (PAN), polyvinylpyrrolidone(PVP), polyisopropylacrylamide (PNIPPAm), a cellulose derivative, astarch derivative, dextran and guar gum, and has a molecular weight ofless than 20,000 Da.
 9. The composition according to claim 7, whereinthe bead- and resin-type polymer resin is a polymer prepared bypolymerizing one or more monomers selected from the group consisting ofstyrene, ethylene, butadiene, acrylonitrile, methylstyrene,terephthalate, ethylene chloride, ketone ether, imide ether, sulfoneether, phthalamide, phenylene ether, phenylene oxide, phenylsulfide,sulfone, urethane, vinylidene fluoride and tetrafluoroethylene, and hasa molecular weight of less than 20,000 Da.
 10. The composition accordingto claim 7, wherein the silica bead has a diameter of 100 nm to 1 mm.11. The composition according to claim 4, wherein the aminium-basedcompound is one or more selected from the group consisting ofN-[(1H-benzotriazol-1-yl)(dimethylamino)methylene]-N-methylmethanaminiumhexafluorophosphate N-oxide,N-[(1H-benzotriazol-1-yl)(dimethylamino)methylene]-N-methylmethanaminiumhexafluoroborate, 2-(3,4-(N-[(1H-benzotriazol-1-yl)(dimethylamino)methylene]-N-methylmethanaminium hexafluorophosphateN-oxide,2-(3,4-dihydro-4-oxo-1,2,3-benzotriazin-3-yl)-1,1,3,3,-tetramethyluronium tetrafluoroborate,O-(3,4,-dihydro-4-oxo-5-azabenzo-1,2,3-triazin-3-yl)-1,1,3,3,-tetramethyluroniumhexafluoroborate,O-(3,4,-dihydro-4-oxo-5-azabenzo-1,2,3-triazin-3-yl)-1,1,3,3,-tetramethyluroniumhexafluorophosphate, 2-(2-oxo-1(2H)-pyridyl-1,1,3,3,-tetramethyluroniumtetrafluoroborate, 2-(2-oxo-1(2H)-pyridyl-1,1,3,3-tetramethyluroniumtetrafluorophosphate, 2-succinimido-1,1,3,3-tetramethyluroniumtetrafluoroborate), 2-succinimido-1,1,3,3-tetramethyluroniumtetrafluorophosphate,N,N,N′,N′-bis(tetramethylene-O-pentafluorophenyluroniumtetrafluoroborate,N,N,N′,N′-bis(tetramethylene-O-pentafluorophenyluroniumhexafluorophosphate, N-[6-trifluoromethyl(1H-benzotriazol-1-yl)(dimethylamino)methylene]-N-methyl methanaminium tetrafluoroborateN-oxide,N-[6-trifluoromethyl(1H-benzotriazol-1-yl)(dimethylamino)methylene]-N-methylmethanaminiumhexafluorophosphate N-oxide,N-[(dimethylamino)-1H-1,2,3-triazolo[4,5,b]pyridin-1-yl]methylene]-N-methylmethanaminiumhexafluorophosphate N-oxide, N-[(dimethylamino)-1H-1,2,3-triazolo[4,5-b]pyridin-1-ylmethylene]-N-methyl methanaminium tetrafluoroborateN-oxide,N-[(dimethylamino)-1H-1,2,3-triazolo[4,5-b]pyridin-1-ylmethylene]-N-methylmethanaminiumhexafluorophosphate N-sulfide,S-(1-oxido-1-pyridinyl)-1,1,3,3-tetramethylthiouroniumhexafluorophosphate,O-[cyano(ethoxycarbonyl)methyleneamino]-N,N,N′,N′-tetramethyluroniumtetrafluoroborate, O-[cyano(ethoxycarbonyl) methyleneamino]-N,N,N′,N′-tetramethyluronium hexafluorophosphate, O-[(dicyanomethylidene)-amino]-1,1,3,3-tetramethyluronium hexafluorophosphate,O-[(dimethoxycarbonylmethylidene)-amino]-1,1,3,3-tetramethyluroniumhexafluorophosphate, N-[(cyano(pyridin-2-yl)methylene aminooxy)(dimethylamino)methylene]-N-methylmethanaminium hexafluorophosphate,2-(5-norbornene-2,3-dicarboximido)-1,1,3,3-tetramethyluroniumtetrafluoroborate, 2-phthalimido-1,1,3,3-tetramethyluroniumtetrafluoroborate, bis(tetra methylene)chloroformamidiniumhexafluorophosphate, (1H-benzotriazol-1-yl)(1-pyrollidinylmethylene)pyrrolidinium hexafluorophosphate N-oxide,1-(1-pyrrolidinyl-1H-1,2,3-triazolo [4,5-b]pyridin-1-ylmethylene)pyrrolidinium hexafluorophosphate N-oxide,O-(3,4-dihydro-4-oxo-1,2,3-benzotriazin-3-yl)-1,1,3,3-bis(tetramethylene)uranium hexafluorophosphate,O-(3,4-dihydro-4-oxo-5-azabenzo-1,2,3-triazin-3-yl)-1,1,3,3-bis(tetramethylene)uranium hexafluorophosphate, N,N,N′,N′-bis(tetramethylene)-O-pentafluoro phenyluronium hexafluorphosphate,N,N,N′,N′-bis(tetramethylene)-S-pentafluorothiophenyluroniumhexafluorphosphate,1-(1-pyrrolidinyl-1H-1,2,3-triazolo[4,5-b]pyridin-1-ylmethylene)pyrrolidinium hexafluorophosphate N-sulfide,N,N,N′,N′-bis(tetramethylene)-O-2-nitrophenyluroniumhexafluorophosphate,N,N,N′,N′-bis(tetramethylene)-O-pentafluorophenyluroniumhexafluorohosphate, O-(benzotriazol-1-yl)-1,1,3,3-bis(pentamethylene)hexafluorophosphate,O-(7-azabenzotriazol-1-yl)-1,1,3,3-bis(pentamethylene)-uroniumhexafluorophosphate, 2-[2-oxo-1(2H)-pyridyl]-1,1,3,3-bis(pentamethylene)uronium tetrafluoroborate, 2-chloro-1,3-dimethylimidazolidiniumhexafluorophosphate, O-(benzotriazol-1-yl)-1,3-dimethyl-1,3-dimethyleneuronium hexafluorophosphate,O-(7-azabenzotriazol-1-yl)-1,3-dimethyl-1,3-dimethyleneuroniumhexafluorophosphate, 2-chloro-1,3-dimethylpyrimidiniumhexafluorophosphate,O-(benzotriazol-1-yl)-1,3-dimethyl-1,3-trimethyleneuroniumhexafluorophosphate,O-(7-azabenzotriazol-1-yl)-1,3-dimethyl-1,3-trimethyleneuroniumhexafluorophosphate, (7-benzotriazol-yl)-1,1,3-trimethyl-1-hexafluorophosphate,(7-azabenzotriazol-yl)-1,1,3-trimethyl-1-phenyluroniumhexafluorophosphate,O-(1H-benzotriazol-1-yl)-1,1-dimethyl-3,3-tetramethylene uraniumhexafluorophosphate,O-(1H-1,2,3-triazolo[4,5-b[pyridin-yl)-1,1-dimethyl-3,3-tetramethyleneuroniumhexafluorophosphate,O-(1H-benzotriazol-1-yl)-1,1-dimethyl-3,3-pentamethylene uraniumhexafluorophosphate,6-chloro-1((dimethylamino)(morpholino)methylene)-1H-benzo triazoliumhexafluorophosphate-3oxide,3-((dimethylamino)-(morpholino)methylene)-1H-[1,2,3]triazolo[4,5-b]pyridiniumhexafluorophosphate,6-trifluoromethyl-1-((dimethylamino)-(morpholino)methylene)-1H-benzotriazoliumhexafluorophosphate-3-oxide,1-((dimethylamino)-(morpholino)) oxypentafluorophenylmetheniminiumhexafluorophosphate,1-[(1-(cyano-2-ethoxy-2-oxoethylideneaminooxy)-dimethylaminomorpholinomethylene)]methanaminium hexafluorophosphate,1-[(1-(dicyanomethyleneaminooxy) dimethylaminomorpholinomethylene)]methanaminium hexafluorophosphate,1-[(1,3-diethoxy-1,3-dioxopropan-2-ylideneaminooxy)dimethylaminomorpholinomethylene)]methanaminium hexafluorophosphate,N-[(cyano(pyridin-2-yl)methyleneaminooxy)(dimethylamino)methylene]-N-morpholinomethanaminiumhexafluorophosphate,1-[(1-(cyano-2-ethoxy-2-oxoethylideneaminooxy)dimethylaminopyrrolodinomethylene)]methanaminium hexafluorophosphate,1-((dicyanomethyleneaminooxy) morpholinomethylene)pyrrolidiniumhexafluorophosphate,1-[(1,3-diethoxy-1,3-dioxopropan-2-yldeneaminooxy)-dimethylamino-pyrrolodinomethylene]]methanaminiumhexafluorophosphate, 1-[(1-(cyano-2-ethoxy-2-oxoethylideneaminooxy)-dimethylamino-pyrrolodinomethylene)]methanaminiumhexafluororphosphate,1-((1-cyano-2-ethoxy-2-oxoethylideneaminooxy)(morpholino)methylene)pyrrolidinium hexafluorophosphate,benzotriazol-1-yloxy-N,N-dimethyl-methanaminium hexachloroantimonate,5-(1H-benzotriazol-1-yloxy)-3,4-dihydro-1-methyl-2H-pyrroliumhexachloroantimonate,5-(7-azabenzotriazol-1-yloxy)-3,4-dihydro-1-methyl-2H-pyrroliumhexachloroantimonate, 1-(1H-benzotriaol-1-yloxy)phenyl-methylenepyrrolidinium hexachloroantimonate,5-(pentafluorophenyloxy)-3,4-dihydro-1-methyl-2H-pyrroliumhexachloroantimonate,5-(succinimidyloxy)-3,4-dihydro-1-methyl-2H-pyrroliumhexachloroantimonate,5-(3′,4′-dihydro-4′-oxo-1′,2′,3′-benzotriazin-3′-yloxy)-3,4-diydro-1-methyl-2H-pyrroliumhexachloroantimonate) and a derivative thereof.
 12. The compositionaccording to claim 4, wherein the phosphonium-based compound is acompound having one or more phosphonium salts in the molecule.
 13. Thecomposition according to claim 4, wherein the phosphonium-based compoundis one or more selected from the group consisting ofbenzotriazol-1-yloxytris (dimethylamino) phosphoniumhexafluorophosphate, bromotris (dimethylamino) phosphoniumhexafluorophosphate, chlorotri (pyrrolidino) phosphoniumhexafluorophosphate, bromotri (pyrrolidino) phosphoniumhexafluorophosphate, chloro-tris(dimethylamino) phosphoniumhexafluorophosphate, benzotriazol-1-yloxytri (pyrrolidino) phosphoniumhexafluorophosphate, [(7-azabenzotriazol-1-yl)oxy]tris-(dimethylamino)phosphonium hexafluorophosphate,[(7-azabenzotriazol-1-yl)oxy]tris-(pyrrolidino) phosphoniumhexaflurophosphate,O-[(cyano-(ethoxycarbonyl)methyliden)-amino]-yloxytripyrrolidinophosphonium hexafluorophosphate,└(6-nitrobenzotriazol-yl)oxy┘tris-(pyrrolidino) phosphoniumhexafluorophosphate,[(6-trifluoromethyl)benzotriazol-1-yl]oxy-tris(pyrrolidino)phosphoniumhexafluorophosphate,[4-nitro-6-(trifluoromethyl)benzotriazol-1-yl]oxy]tris(pyrrolidino)phosphonium hexafluorophosphate, (6-chloro-benzotriazol-1-yloxy)tris(pyrrolidino)-phosphonium hexafluorophosphate,N′,N′,N′,N′-bis(tetramethylene)-O-pentafluoro phenyluroniumhexafluorophosphate, (pyridyl-2-thio)tris (pyrrolidino)-phosphoniumhexafluorophosphate,[(3,4-dihydro-4-oxo-1,2,3-benzotriazin-3-yl)oxy]tris-(pyrrolidino)phosphoniumhexafluorophosphate,[(3,4-dihydro-4-oxo-5-azabenzo-1,2,3-triazin-3-yl]tris-(pyrrolidino)phosphonium hexafluorophosphate and a derivative thereof.
 14. Thecomposition according to claim 4, wherein the enzyme istransglutaminase, sortase A, tyrosinase, laccase/peroxidase or lysyloxidase/amine oxidase.
 15. The composition according to claim 1, whichis for enhancing the strength of a hair, scalp, skin, finger/toe nail,leather or fiber protein.
 16. The composition according to claim 1,wherein the aminosilane compound is contained at 0.000001 to 30 parts byweight with respect to 100 parts by weight of the total composition. 17.The composition according to claim 1, wherein the reaction mediator iscontained at 0.001 to 20 parts by weight with respect to 100 parts byweight of the total composition.
 18. A care product comprising thecomposition for enhancing protein strength of claim 1.